UJI PEWARNA GRAM BAKTERI ESCHERICHIA COLI PADA JAJANAN CILOK DI DUA SEKOLAH DASAR KOTA JAMBI PADA TAHUN 2024

Abstract

Gram staining tests carried out on E.coli bacteria obtained gram-negative bacteria with a short rod shape. The results of the isolation of bacteria into EMB specific media were then incubated for 24 hours at a temperature of 35-360C, then a colony was formed, the samples used in this study were 2 samples of cilok snacks taken from two elementary schools in the city of Jambi. The tools used in this study are beker glasses (250mL and 500 mL), erlenmeyer (250mL and 500mL), measuring tubes (100mL and 10 mL), test tubes, test tube racks, petri dishes, bunsen, spatula, tweezers, pipettes, ose (round and needle), L rod, lighter, tip (1000? and 100?), micropipette (1000?L and 100?L), blender, autoclave, oven, incubator, refrigerator, laminar, vortex, scale, hot plate, magnetic stirr, tissue, cotton, heat-resistant plastic, handscoon, mask, solution for Gram staining (KKU, lugol, 90% alcohol, safranin), microscope, immersion oil, waste paper, durham tube. The materials used are snack samples, EMBA (Eosin Methylene Blue Agar), Nutrient Agar (NA), Nutrient Broth (NB) media, aquadest, 70% alcohol and 96% alcohol, aluminum foil, cotton, tissues, newspapers, matches. Based on the results of this study, it can be concluded Based on the results of the study, namely in two schools in Pall Merah and Kota Baru sub-districts, cilok food containing Escherichia coli is suspected to be 20%.

Kata Kunci: Cilok, Bakteri, Escherichia coli